How the DNA is Obtained from mRNA? (2 Methods)
In shotgun method gene cloning is a general one and not specific. As the genes to be cloned are not identified and isolated before, the chances of inserting a particular gene by this method are very rare.Another specific method of isolating gene is to first obtain the messenger RNA transcribed by that particular gene and then obtain DNA from mRNA. DNA from mRNA can be obtained by the following two methods: Image Source: i.huffpost.com 1. Hybridization method: ADVERTISEMENTS: When DNA transcribes and forms mRNA a complex is formed. The hybridization method depends on this mechanism.At the first step the total DNA from an organism is isolated and treated with heat or alkali to convert it into a single stranded form.These single strands are then mixed with the specific mRNA obtained…