Useful Notes on the Preparation and Preservation of Antisera
A complete antisera is the serum of an animal, immunized by injection of serum from other animal.Antisera in use are available commercially but can be prepared easily in laboratory from collected blood of immunized donors.When collecting blood for antisera, collect blood in a sterile container without anticoagulant. Incubate at 37QC for 1 hr. to get good clot retraction. ADVERTISEMENTS: Leave the clotted blood at 4oC overnight to allow absorption of cold agglutinins on the red cells. Centrifuge the container and separate the serum in a second sterile container.If plasmapheresis is carried out, add one ml of 1 M CaCl2.6 H2O for every 100 ml of plasma to be defibrinated. Incubate at 37oC for 15 to 30 min. allowing clot to form and retract. Separate the clear serum.To prevent bacterial contamination,…